منابع مشابه
Sequence of the rat factor VIII cDNA.
Factor VIII acts as an essential compound of the tenase complex of the coagulation system. Herein we report the cDNA of the rat factor VIII. The rat cDNA comprises 6777 nucleotides and encodes a protein of 2258 amino acids, 61 amino acids less than mouse and 92 amino acids less than human factor VIII. The over-all identity compared to human cDNA is 61% on the cDNA and 51% on the amino acid leve...
متن کاملCloning and sequence analysis of cDNA for rat angiotensinogen.
A mixture of tetradecamer oligodeoxyribonucleotides complementary to the codons specifying the carboxyl-terminal sequence, Ile-His-Pro-Phe-His, of angiotensin was chemically synthesized as two pools and used for the isolation of a cDNA clone specific for angiotensinogen from a cDNA bank of rat liver mRNA sequences. The two pools (oligo 1 and oligo 2), each containing 24 oligodeoxyribonucleotide...
متن کاملCloning and sequence analysis of a cDNA encoding rat preprocholecystokinin.
Poly(A) RNA was isolated from a rat medullary thyroid carcinoma that exhibited high levels of immunoreactive cholecystokinin (CCK). Double-stranded cDNA was synthesized from the poly(A) RNA and inserted into the Pst I site of pBR322. Bacterial colonies containing CCK cDNA were identified using the hybridization probe d(T-C-C-A-T-C-C-A-N-C-C-C-A-T-G-T-A-G-T-C). The sequence of the probe was dedu...
متن کاملA cDNA sequence encoding cytoskeletal gamma-actin from rat.
We have isolated and sequenced three clones from rat stomach or kidney cDNA libraries. These clones encode the cytoskeletal isoform of 7-actin as determined by comparison with published protein sequence (1), as well as with cDNA sequences from the human (2) and mouse (3). The sequence of the longest kidney clone, PRC7A-2, is shown below. It displays poly 'G' and ' C homopolymeric tails, 12 nucl...
متن کاملRat hypoxanthine phosphoribosyltransferase cDNA cloning and sequence analysis.
We have determined the nucleotide sequence of the rat hprt (hypoxanthine phosphoribosyltransferase; EC 2.4.2.8.) mRNA coding region and of adjacent, untranslated 5' and 3' mRNA, and we have designed an oligonucleotide primer pair for efficient PCR amplification of the rat hprt coding region. These sequence data and rat-specific primer pair will aid workers interested in coupling well-developed ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.14.4251